Improvement of thermostable productivity ?-amylase from local isolate Bacillus licheniformis H14.

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Single Step Purification of Novel Thermostable and Chelator Resistant Amylase from Bacillus Licheniformis RM44 by Affinity Chromatography

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

Single Step Purification of Novel Thermostable and Chelator Resistant Amylase from Bacillus Licheniformis RM44 by Affinity Chromatography

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

BACKGROUND Some strains of Bacillus licheniformis have been improved by target-directed screening as well as by classical genetic manipulation and used in commercial thermostable alpha-amylase and alkaline protease production for over 40 years. Further improvements in production of these enzymes are desirable. RESULTS A new strain of B. licheniformis CBBD302 carrying a recombinant plasmid pHY...

Thermostable a-Amylase of Bacillus stearothermophilus

Some general and physical properties and the’ amino acid composition of the thermostable cr-amylase of Bacillus sbarothermophilus have been described in previous papers (l-3). In an effort to further correlate the thermostability of this enzyme with its molecular organization, a study of the structure of this protein has been undertaken. In this paper are reported the results of an investigatio...